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. 2016 Nov 2;6:35846. doi: 10.1038/srep35846

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Copyright © 2016, The Author(s)

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Genes that were statistically relevant (P < 0.05) and with a ∆RPKM between Mn-deficient and Mn-sufficient plants greater than the median of the RPKM values of all robustly expressed genes were referred to as differentially expressed (DE transcripts). For proteins, a change in the abundance in the same direction in all three biological replicates and a P-value < 0.05 (Z-test) was used as criteria to define differential expression (DE proteins).