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. 2016 Nov 2;6:36412. doi: 10.1038/srep36412

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The protein sequences of RoxP homologs were extracted from all available P. acnes genomes. The tree, generated by the minimum-evolution algorithm in MEGA version 5.2, illustrates that RoxP is highly conserved among the vast majority of P. acnes strains. Most strains (58) have an identical homolog, and 33 strains have highly similar homologs (99% identity). A subgroup of 11 RoxP homologs belonging to strains of P. acnes type II, type III, and P. humerusii are more distant (83% identity). Some genomes, such as JCM strains, encode a truncated RoxP. The bar represents the genetic distance.