Figure 4. RoxP has antioxidant properties.

(a) RoxP was incubated with preformed ABTS-radicals and changes in absorbance was measured over time (0–18 min) and summarized as % inhibition (b). Culture supernatant from wildtype P. acnes (c) and ΔroxP-mutant P. acnes lacking RoxP (d) were incubated with hemoglobin and absorbance was measured over time (0–5 h). Black lines represent the starting absorbance, and blue lines changes over time. All samples were analyzed at a maximum absorbance of 0.6 through dilutions, and are reported as measured absorbance multiplied by dilution factor. All experiments were performed at least three times, and are presented as representative figures.