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. 2016 Nov 2;6:36201. doi: 10.1038/srep36201

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(a,b) TRAP staining show excess osteoclasts (stained in red) in 4-week-old experimental animals. (c) Osteoclast numbers per mm bone surface in cancellous bone of L2-4 vertebra of 4-week-old control (n = 6) and experimental (n = 6) littermates and representative histological photomicrographs of vertebral trabecular bone. (d) Histomorphometric analysis of longitudinal decalcified sections of L2–L4 vertebrae from 4-week-old female mice (n = 6 mice per group) showing no difference in bone area per tissue area (BA/TA) between control and experimental animals. (e) Quantification of TRAP-positive cells generated from bone marrow cells cultured with M-CSF and RANKL for 5 days and representative microphographs of the culture dishes. Bars, Mean ± SD; *P < 0.05. Scale bar, 1000 μm.