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. 2016 Nov 2;6:36201. doi: 10.1038/srep36201

Figure 6. WNT3A induces osteoclast apoptosis via the canonical Wnt signaling pathway.

Figure 6

(a) Number of TRAP-positive cells generated from non-adherent bone marrow cells from C57BL/6 control mice cultured with M-CSF, RANKL and either vehicle (veh) or different doses of WNT3A recombinant protein as indicated. (b) TUNEL assay in mature osteoclasts generated from bone marrow cells from Ctnnb1f/f;CtsKCre mice and their respective littermate controls, cultured with M-CSF and RANKL for 5 d and treated with either veh or WNT3A (50 ng/ml) for 24 h. (c) Caspase 3 activity, expressed in arbitrary fluorescent units, in mature osteoclasts generated from non-adherent bone marrow cells from wild-type mice, and treated with veh, WNT3A (50 ng/ml) or DKK1 (1 μg/ml) for 24 h. Bars, Mean ± SD; *P < 0.05.