Figure 1. Conservation of Dnmt2, Mettl4 and Tet in Ae. aegypti.

(A) An analysis of the Ae. aegypti peptide database version 3.3 revealed the presence of single homologues for Dnmt2, Mettl4 and Tet, respectively. Conserved domains are shown as colored boxes. Dnmt1: orange - DMAP1 binding domain, red - replication foci domain, blue - CXXC zinc finger domain, green - bromo adjacent homology domain, purple - catalytic domain. Dnmt2: purple - catalytic domain. Dnmt3: green - PWWP domain, blue - zinc finger domain, purple - catalytic domain. Mettl4: magenta - catalytic domain. Tet: blue - CXXC zinc finger domain, pink-catalytic domain. Accession numbers are provided below the protein symbols. n.d., not detected. (B) Multiple sequence alignment of the DNA methyltransferase motifs of AaDnmt2 and the corresponding sequences of several Dnmt2 homologs with experimentally validated C38 tRNA methyltransferase activity. Black background denotes 100% identity, dark grey background 80–99% identity and grey background 60–79% identity. The catalytic motifs are highlighted in red, the Dnmt2-specific CFT motif³⁴ is highlighted in green. For additional details, see Fig. S1.