Figure 6.

Intracellular distribution of cRGD-conjugated disulfide-crosslinked PIC micelles. Polyplex micelles loading Cy5-labeled pDNA (red) and CT-B Alexa Fluor 488 conjugate, a marker for the lipid rafts and the caveosomes (green), were incubated with HeLa cells for 1 h, washed and reincubated for 11 h. The cell nuclei were stained with Hoechst 33342 (blue). (a) CLSM images of RGD (−) micelles (left) and RGD (+) micelles (right). The scale bars represent 20 μm. (b) Quantification of Cy5-labeled pDNA colocalized with CT-B in the inner-cytoplasm. Error bars represent SEM (n=10). (Reprinted with permission from [72] ©2008 The American Society for Pharmacology and Experimental Therapeutics.)