Figure 9.
SK-Hep1 cell adhesion on PLA/PEG scaffolds. (A) SK-Hep1 cells cultured on (a) PLA/PEG2 scaffold pretreated with type I collagen solution, (b) on untreated PLA/PEG2 scaffold, (c) on PLA/PEG3 scaffold pretreated with type I collagen solution and (d) on untreated PLA/PEG3 scaffold. The cells were stained with a solution of phalloidin-FITC and EtBr. Panels (A) a'–d' are magnifications of the squared areas in (A) a–d. Bar = 50 μm. (B) SK-Hep1 cell morphology when cultured on plastic (a), on PLA/PEG2 (b) and PLA/PEG3 (c). Cell plasma membrane surfaces were stained with a primary antibody against β1-integrin and secondary FITC-conjugated antibody to reveal the cell adhesion and spreading on different surfaces. Bar = 20 μm.