Figure 6.

(a) Elements comprising the framework: parental cloning vectors harboring a custom multiple cloning site (MCS) of optimal restriction enzyme sites, a library of commonly used synthetic genetic components designed to exclude the restriction sites, and a repository of assembled constructs that includes synthetic modules, intermediates and circuits. (b) Generalized workflow for constructing and modifying synthetic gene networks, which prioritizes and streamlines the iterative process of arriving at functional networks and modules. Reprinted by permission from Macmillan Publishers Ltd: Nature Methods [40], copyright 2012.