Figure 4. Dual inhibition of VEGF‐A and ANG‐2 reduced the number of IBA‐1⁺ macrophages in the model of sCNV in JR5558 mice.

• A
  Bar graph of number of IBA‐1⁺ cells in the retina of mice treated with IgG control, anti‐VEGF‐A, anti‐ANG‐2 (all 5 mg/kg IP), or bispecific anti‐VEGF‐A/ANG‐2 (at 10, 5, and 3 mg/kg) in the late interference model at D60. Error bars show SEM with n = 6 animals per group. All significant differences are shown by * after one‐sided ANOVA (P < 0.0008) and Tukey's multiple t‐test. IgG control is significantly different vs. anti‐VEGF‐A/ANG‐2 low (*, P < 0.0134), mid (**, P < 0.0078), and high (**, P < 0.0042); furthermore, anti‐ANG‐2 is significantly different vs. anti‐VEGF‐A/ANG‐2 mid (*, P < 0.0353).
• B–G
  (B) IgG control, (C) anti‐VEGF‐A, (D) anti‐ANG‐2, (E–G) anti‐VEGF‐A/ANG‐2 low, mid, and high: representative micrographs of whole‐mount eyecup preparations stained with isolectin‐B4 positive (green) to stain vessels and anti‐IBA1 (red) to stain for macrophages show a decrease in both absolute numbers and clustering of subretinal IBA‐1‐expressing cells associated with neovascular complexes in anti‐VEGF‐A/ANG‐2 treatment groups. Scale bar, 200 μm.