Figure 7. Binding of CrossMAbs with modification of the Fc part to FcγRI, II, and III, as well as to FcRn in SPR interaction assays.

• A–D
  Time‐dependent binding of RG7716 is shown in blue, anti‐VEGF‐A/ANG‐2 without modification of the Fc part in red (wild‐type), anti‐VEGF‐A/ANG‐2 with modification of the FcRn binding site only in green (FcRn⁻), and anti‐VEGF‐A/ANG‐2 with modification of the FcγR binding in pink (FcγR⁻). Panel (A) shows SPR binding profile over time to immobilized FcγRI, (B) to immobilized FcγRII, (C) to immobilized FcγRIII, and (D) to immobilized FcRn. SPR, surface plasmon resonance.
• E
  Summary data table of binding data for RG7716 in comparison with anti‐VEGF‐A/ANG‐2 CrossMAbs without modifications in the Fc part.