Figure 2. Am80‐GCSF induces both higher bactericidal activities and transcription of RA‐target genes that regulate neutrophil differentiation in primary human leukemic specimens.

1. Fresh bone marrow (BM) mononuclear cells were collected from acute myeloid leukemia (AML) patient. Proliferation (i) and ROS production (ii) were analyzed after cells were treated for 4 days.
2. Fresh BM mononuclear cells collected from AML patient were treated for up to 6 days. Transcription of RA‐target genes was analyzed with qRT–PCR at day 2, 3, and 6 (i). Both proliferation and ROS production were assessed at day 3 in parallel (ii, iii).

Data information: Data are shown as mean ± SD and represent at least two independent experiments with similar results. *P < 0.05; **P < 0.01; ***P < 0.001 (Student's unpaired two‐tailed t‐test). Exact P‐values are provided in Appendix Table S4.