Figure 5. Am80‐GCSF coordinates myeloid expansion with granulocytic differentiation to generate large amounts of functional neutrophils that reduce infection in CCIN mice.

• A
  After 48 h of CPA injection, mice were treated with low doses of Am80 and/or GCSF for 3 days. Mice were infected with 9 × 10⁶ CFU of S. aureus through intravenous injection on day 4 and sacrificed 16 h post‐infection. Control mice without CPA.
• B
  Vetscan counting PB leukocytes.
• C
  Bacterial killing by PB neutrophils was evaluated 3 and 16 h post‐infection (i, ii), using blood agar analysis of extracellular viable bacteria.
• D, E
  Neutrophil recovery and morphologic differentiation induced by low doses of Am80 and/or GCSF in BM (D) and PB (E). White arrows indicate neutrophil nuclear segmentation.

Data information: Data are shown as mean ± SD and represent three independent experiments with similar results. *P < 0.05; **P < 0.01; ***P < 0.001 (Student's unpaired two‐tailed t‐test). Exact P‐values are provided in Appendix Table S4.