Figure 6. Am80‐GCSF reduces infection‐related mortality in CCIN mice undergoing perpetual systemic intravenous bacterial infection throughout a full cycle of mouse CCIN.

1. Survival model #1 with low‐dose treatment after 4 h of CPA injection. Mice were infected with 5 × 10⁶ CFU of S. aureus via intravenous injection on day 3. Moribund mice were euthanized and recorded as deceased on the day of euthanasia. Control mice without CPA.
2. Survival of CCIN mice was calculated with both Kaplan–Meier plots and log‐rank test on day 9 since CPA injection (i) as well as assessed 6 days post‐infection (ii). Data represent three independent experiments with similar results.
3. Gram and Giemsa stains analyzed neutrophil phagocytosis and bacterial clearance in mice surviving on day 9. White arrows indicate phagocytosed S. aureus in neutrophils.
4. Survival model #2 with low‐dose treatment after 24 h of CPA injection. Other schedules/procedures were the same to those described in (A).
5. Survival of CCIN mice was calculated with both Kaplan–Meier plots and log‐rank test on day 9 since CPA injection (i) as well as assessed 6 days post‐infection (ii). Data represent two independent experiments with similar results.
6. Survival model #3 with medium‐dose treatment after 24 h of CPA injection. Other schedules/procedures were the same to those described in (A).
7. Survival of CCIN mice was calculated with both Kaplan–Meier plots and log‐rank test on day 9 since CPA injection (i) as well as assessed 6 days post‐infection (ii). Data represent two independent experiments with similar results.