Figure 7. Am80‐GCSF prevents myeloid overexpansion while generating sufficient numbers of functional neutrophils during “emergency” granulopoiesis in CCIN mice.

• A, B
  Neutrophil recovery and morphologic differentiation were assessed in BM (A) and PB (B) of CCIN mice surviving on day 9. White arrows indicate neutrophil nuclear segmentation.
• C
  PB neutrophil production and nuclear segmentation were assessed in control and Am80‐GCSF mice surviving on day 13 (i, ii). White arrows indicate neutrophil nuclear segmentation. The increased spleen size and weight on day 9 in Am80‐GCSF mice dropped to the levels similar to those in control mice on day 13 (iii, iv).
• D
  Significant loss of body weight occurred in GCSF mice on day 9.
• E
  Ultrastructure analysis of neutrophils collected from mice surviving on day 11. Neutrophils from GCSF mice showed less granules (i, images 1 versus 2 or 3), more intracytoplasmic vacuoles (i, ii), and inner nuclear membrane dilation (i, iii). Pg: primary granules; Sg: secondary granules.

Data information: Data are shown as mean ± SD and represent at least two independent experiments with similar results. *P < 0.05; **P < 0.01; ***P < 0.001 (Student's unpaired two‐tailed t‐test). Exact P‐values are provided in Appendix Table S4.