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A, B
Arabidopsis thaliana plants were inoculated with wild‐type, XccbphP, pXccBphP, or pC13S bacterial strains cultured prior to inoculation under (A) normal laboratory illumination or (B) under light or dark conditions. After 1, 2, or 3 d.p.i., CFU per plant mg were determined (n = 3 replicates). Data presented here derive from more than four independent experiments.
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C, D
(C) Stomatal closure in the presence of bacterial strains or not (untreated) after 1 h under light conditions. (D) Stomatal opening after 3 h of incubation with bacterial strains or not (untreated) in the dark. A control treatment without bacteria and illuminated was included (Light). (C, D) Stomatal apertures were recorded (n = 80 replicates). Data are representative of two independent experiments.
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E
Arabidopsis thaliana leaves were inoculated with wild‐type, XccbphP, pXccBphP, and pC13S strains, stained for callose deposits and observed by fluorescence microscopy. MgCl2 buffer (untreated) and flg22 peptide were used as negative and positive controls, respectively. Top panel: representative pictures of three independent experiments. Scale bar represents 200 μm. Bottom panel: the number of callose deposits per field of view (0.45 mm2) were determined (n = 8 replicates). Data are representative of two independent experiments.
Data information: (A–E) Values are expressed as mean ± s.e.m. Statistical analysis was performed by a two‐tailed Mann–Whitney test (*
< 0.001).
