Table 2.

Motional Parameters for SkMM and SMM

sample	motional param	regulatory domain		catalytic domain		buffer	ref
		UPa	Pa	UP	P
SMM filamentb	corr time (μs)	441 ± 79 (11)c (Cys 108 RLC)	408 ± 54 (3)	24 ± 6 (7) (Cys 717)	17 ± 7 (2)	10 mM MgCl2, 15 mM Tris pH 7.5, 0.1 mM EGTA, 50 mM KCl, 4 °C	this work
	cone angle	24 ± 9° (Cys 108 RLC)	36 ± 7°	42 ± 4° (Cys 717)		10 mM MgCl2, 10 mM MOPS pH 7.5, 0.1 mM EGTA, 50 mM KCl, 4 °C	this work
skMM filamentb	corr time (μs)	40 ± 3 (3) (Cys 154 RLC)		10 ± 2 (3) (Cys 707)		40 mM KCl, 10 mM MOPS, pH 7.0, 4 °C	this work
		36 ± 12 (Cys 177 ELC)		6.9 ± 1.5 (Cys 707)		40 mM KCl, 10 mM MOPS, pH 7.0, 4 °C	27
		26.2 ± 2.0 (4) (Cys 177 ELC)	16.9 ± 8 (4)			50 mM MOPS, 0.1 mM DTT, pH 7.0, 4 °C	28
	cone angle	29 ± 8° (Cys 177 ELC)		51 ± 5° (Cys 707)		120 mM KCl, 5 mM MgCl2, 1 mM EGTA, 25 mM MOPS, pH 7.0, 12 °C	30
		27 ± 6° (Cys 154 RLC)				10 mM MgCl2, 15 mM Tris pH 7.5, 0.1 mM EGTA, 50 mM KCl, 4 °C	this work
SMM monomer (6S)	corr time (μs)	4.4 ± 0.5 (3) (Cys 108 RLC)		3.4 ± 0.2 (4) (Cys 717)		0.5 M KCl, 10 mM MOPS pH 7.0, 0.1 mM EDTA, 4 °C	this work
skMM monomer	corr time (μs)	4.1 ± 0.5 (3) (Cys 154 RLC)		4.4 ± 0.6 (3) (Cys 707)		0.6 M KCl, 20 mM MOPS, pH 7.0	27
skMM rods	corr time (μs)	31000 μs (rod domain)				40 mM KCl, 10 mM MOPS pH 7.0, 4 °C	27

^aUP, unphosphorylated; P, phosphorylated.

^bSMM are side-polar, skMM filaments are bipolar.

^cNumber in parentheses is the number of independent measurements.