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. 2016 Nov 2;6:36312. doi: 10.1038/srep36312

Figure 1. A2MC2-P90 induces synthesis of interferons.

Figure 1

(A) Interferon bioassay in Vero cells. Dilutions of cell culture supernatant of MARC-145 cells infected with A2MC2-P90 (A2P90) or A2MC2-P9 (A2P9) were used to treat Vero cells. Treatment with 1000 U IFN-α was included as a control. At 12 h after the treatment, the Vero cells were inoculated with NDV-GFP. At 24 h post-inoculation of NDV, the cells were observed under fluorescence microscopy. (B) Elevation of RIG-I in Vero cells treated with A2MC2-P90 supernatant. The Vero cells were treated with culture supernatant from MARC-145 cells infected with VR-2385 (VR) or A2MC2-P90 (A2) and harvested 24 and 48 h post treatment for immunoblotting. (C) Treatment with A2MC2-P90 supernatant leads to elevation of RIG-I and MDA5 expression in Vero cells detected by real-time PCR. Relative levels of transcripts are shown in folds in comparison to treatment with supernatant from mock-infected MARC-145 cells. Significant difference from treatment with supernatant of mock-infected cells is denoted by “*”, which indicates P < 0.05 and “***” for P < 0.001.