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. 2016 Nov 2;6:36312. doi: 10.1038/srep36312

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Copyright © 2016, The Author(s)

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(A) Multi-step growth curve of A2MC2-P90 in MARC-145 cells. The cells were inoculated with A2MC2 virus at a multiplicity of infection (MOI) of 0.01, 0.1 or 1 per cell. Inoculation of A2MC2-P9 at an MOI of 0.1 was included as a control. Virus yields at different time points after inoculation were titrated by an immunofluorescence assay. Error bars represent variation of three repeated experiments. “***”Denotes significant difference (P < 0.001) in virus yields between A2MC2-P90 and A2MC2-P9. (B) Plaque assay in MARC-145 cells. The cells were infected with diluted A2MC2-P9 and A2MC2-P90 and overlaid with agarose. Plaques were observed 4 days post-infection and photographed for comparison.