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. 2016 Nov 2;6:35762. doi: 10.1038/srep35762

Figure 2. Staurosporine increases sphingomyelin contents in both the exofacial leaflet of the plasma membrane and intracellular compartments.

Figure 2

(a) Confocal images of CHO cells treated with DMSO, 50 nM STS or 50 nM STS + 15 μM fumonisin B1 (FB1) for 24 hr labelled with MBP-GFP-NT-Lysenin (Lys) protein. Bar, 10 μm. (b) Binding of MBP-GFP-NT-Lys protein to the exofacial leaflets of the plasma membrane in DMSO, STS or STS + FB1 treated CHO cells. Data are means ± SEM (n = 3). ***p < 0.001. MFI; mean fluorescence intensity. (c) Confocal images of DMSO, STS or STS + FB1 treated CHO cells stained with MBP-GFP-NT-Lys protein after membrane permeabilization by 0.05% (w/v) saponin. Bar, 10 μm. The value of fluorescence intensity of MBP-GFP-NT-Lys was shown in images. Data are means ± SEM (50 cells from three independent experiments).