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. Author manuscript; available in PMC: 2016 Nov 2.
Published in final edited form as: Exp Neurol. 2015 Jun 6;271:262–278. doi: 10.1016/j.expneurol.2015.06.007

Figure 8.

Figure 8

Regeneration of CGRP+ axons with combined treatment of neurotrophic factors or GDNF-family co-receptors. CGRP+ axons exhibit robust ectopic regeneration and mistargeting occupying most of the dorsal horn laminae in animals co-expressing artemin with either NGF (A, a) or GFRα3 (B, b). Magnified images of the dorsal horn for each treatment group in the upper panel (a & b). C, Co-expression of GDNF with artemin did not alter the ability of artemin to topographically target regenerating CGRP+ axons. D, Co-expression of GDNF with GFRα1 induce very little, if any, regeneration of CGRP+ axons. Magnified images of C & D in upper panel (c and d, respectively). Scale bar = 300 μm (A, B, C, & D) or 100 μm (a, b, c, & d). E, Quantification of the area occupied by CGRP positive axons in the dorsal horn. For the quantification, lamina specific regions of the dorsal horn were measured as described in figure 2. Compared to artemin (from figure 2) there was a significant increase in axon occupying area in laminas III-VI in both artemin+NGF and artemin+GFRα3 groups (*p<.05, Tukey’s posthoc test). Also lamina IV showed a significant increase in CGRP+ axon occupying area in artemin+NGF group (n=6) compared to artemin+GFRα3 (n=6) (δ p<0.05, Tukey’s posthoc test). Artemin+GDNF (n=7) and GDNF+GFRα1 (n=5) had no effect on topography of CGRP+ axons.