Figure 2. L. pneumophila prevents proliferation of A. castellanii through the Dot/Icm secretion system.

A. castellanii ATCC 30010 were infected with wild type (WT) L. pneumophila Paris, heat-killed (65 °C) and ∆dotA L. pneumophila Paris at a MOI of 20. Sixteen, twenty-four and forty-eight hours after infection, cells were harvested for counting. Results are average of three independents experiments and errors bars represent the standard error of the mean. Cell numbers are relative to uninfected cells at 16 h. The asterisks indicate conditions that are significantly different compared to uninfected cells (***p < 0.001).