Figure 6. L. pneumophila induces a down-regulation of the A. castellanii cell cycle related protein CDC2b.

A. castellanii ATCC 30010 were infected with L. pneumophila Paris at a MOI of 20. RT-qPCR for CDC2b mRNA extracted was performed just after the end of infection (0 h) and 2 h latter (2 h). Expression levels were normalized to the 18S mRNA. CDC2b levels are presented as levels relative to the uninfected condition. Results are average of three independent experiments and errors bars represent the standard error of the mean (±SEM). The asterisks indicate a significant difference between uninfected and infected conditions (***p < 0.001).