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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Reprod Toxicol. 2016 May 18;65:436–447. doi: 10.1016/j.reprotox.2016.05.007

Figure 4.

Figure 4

Figure 4

A: Western blot analysis of SIRT1 expression in whole hippocampus homogenates from 6 month old offspring previously exposed GD1-PD21 to cigarette smoke. Hippocampus homogenates from CSE offspring at age 6 months exhibited a decrease of approximately 50% in expression of the metabolic regulatory protein SIRT1 when compared to Sham exposed (*p<0.05; n=4 per group). Long horizontal lines indicate mean signal intensity, with the standard deviation represented by the shorter horizontal bars.

B: Western blot analysis of NAMPT expression in whole hippocampus homogenates from 6 month old offspring previously exposed GD1-PD21 to cigarette smoke. Hippocampus homogenates from CSE offspring at age 6 months exhibited a decrease of approximately 11% in expression of the metabolic regulatory protein NAMPT when compared to Sham exposed (p<0.07; n=4 per group). Long horizontal lines indicate mean signal intensity, with the standard deviation represented by the shorter horizontal bars.