Fig. 4.

Influence of TR SUMOylation-defective mutants in a mammalian one-hybrid assay. a Schematic overview of different Gal4-DBD-TR fusion proteins. Numbers indicate the position of the diverse functional domains within the protein sequence. * p < 0.05 relative to unstimulated, corresponding wild-type levels; ^# p < 0.05 relative to stimulated wild-type (wt) levels. b, c Expression vectors of the indicated Gal4-TR fusion proteins (wild-type or mutated versions) together with an UAS-Luc reporter vector have been transfected into HepG2 cells and stimulated with T₃. c The induction rates ± T₃ are indicated. Expression rates are displayed in relation to those of the corresponding wild-type Gal4-TR version without T₃ stimulation ± SD.