Fig. 6.
ChIP and Re-ChIP analysis of TRβ on mGPDH and TSHα gene promoters. mGPDH or TSHα promoter constructs were transfected together with Flag-TRβ wild-type (wt) or double mutant Flag-TRβ(K50,443R) into HepG2 cells. a, b Chromatin was immunoprecipitated with an anti-Flag antibody and TR-bound DNA was quantified by qPCR. After the first immunoprecipitation the antigen-antibody complex was released and a second immunoprecipitation was performed using an anti-SRC-1 antibody (c, d) or an anti-NCoR antibody (e, f). DNA occupancy was calculated relative to the occupancy of the TR wild-type version without T3 stimulation ± SD. * p < 0.05 relative to unstimulated wild-type levels; # p < 0.05 relative to unstimulated wild-type levels.