Fig. 3.

T cells stimulated with high-dose peptide are not anergic. (A) At 72h after stimulation in the presence (black bars) or absence (white bars) of exogenous IL-2 (25U ml⁻¹), the proliferative response of the T-cell clone was analyzed by [³H]TdR incorporation. **P < 0.01. (B) The T-cell clone was cultured with BMDCs and different doses of OVA-p (0, 10, 1000nM) without (white bars) or with CsA, 0.25 or 2.5ng ml⁻¹, gray and black bars, respectively. At 72h, proliferation was determined by [³H]TdR incorporation. **P < 0.01. Data are shown as the mean ± SD of triplicate cultures and similar results were obtained in more than three additional experiments. (C) Anergy-related genes (19) were extracted from our microarray data. The heatmap shows the fold change of the expression levels relative to 0h.