Fig. 5.

Inhibition of MEK/ERK signaling restores proliferation at high dose peptide. (A–E, G) The T-cell clone was cultured with BMDCs and OVA-p (0, 10, 1000nM) for 72h with Akti 1/2 (A), Rapamycin (B), SB203580 (C), JNK inhibitor II (D), U0126 (E) and FR180204 (G). Cultures were pulsed with [³H]TdR for the last 6h of the 72h incubation. Data are showed as the mean ± SD of triplicates and similar result were obtained in more than three additional experiments. (F) The T-cell clone was stimulated with a high dose of OVA-p (1000nM) with U0126 (5 μM), and fold expansion was calculated. Data are the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01.