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. 2016 Oct 27;36(5):e00399. doi: 10.1042/BSR20160234

Figure 4. UTI inhibited the levels of RANK-positive cells in a dose-dependent manner in LPS/PMMA-induced Raw264.7 cells.

Figure 4

The cells were stimulated with alone LPS (200 pg/ml) or treated with or without various concentration (500 and 5000 units/ml) of UTI for 3 h prior to being treated with 0.5 mg/ml PMMA with adherent LPS for 24 h. (A) Flow cytometry was performed to examine the levels of RANK-positive cells in Raw264.7 cells from each group. (B) The ratio of RANK-positive cells among total nonadherent Raw264.7 cells was quantified by the labelled M1 line. Data are presented as mean ±S.E.M. from three independent experiments.*, P<0.05 and **, P<0.01 compared with control; #, P<0.05 and ##, P<0.01 compared with PMMA group.