Figure 8. UTI suppressed the expression levels of proinflammatory cytokines in a dose-dependent manner in PMMA-induced murine osteolysis model.

Macrophages were isolated from the femurs of murine osteolysis model at the end of the experimental time line, and the conditioned medium from each group were collected, filtered through a 0.22 mm filter, and then assayed for some inflammation-related cytokines. MMP-9 (A), TNF-α (B), IL-6 (C), RANK (D) and cathepsin K (E) were quantified by ELISA. Data are presented as mean ± S.E.M. from three independent experiments. ^*, P<0.05 and ^**, P<0.01 compared with control; ^#, P<0.05 and ^##, P<0.01 compared with PMMA group.