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. 2016 Feb 19;41(4):327–334. doi: 10.1111/phen.12134

Figure 1.

PHEN-12134-FIG-0001-b

Siesta sleep after feminization and masculinization of mushroom bodies in Drosophila melanogaster. Box plots showing siesta sleep in flies carrying the 121Y‐GAL4 transgene driving (A) UAS‐tra (feminization of males) and (B) UAS–tra2 (masculinization of females). In each panel, the three boxes on the left show sleep in females and the three boxes on the right (shaded grey) show sleep in males. The data represent siesta sleep for the GAL4/UAS genotypes (G4/U, white, n ≥ 20 for all GAL4 lines; males and females) and the single transgene control genotypes (GAL4/+, G4, light grey; UAS/+, U dark grey) for both sexes. Asterisks represent experimental genotype (GAL4/UAS) significance levels compared with control genotypes (GAL4/+ and UAS/+). Nonparametric post‐hoc tests were performed (*P < 0.05, **P < 0.01). The line within each box represents the median siesta sleep (min) averaged over 4 days, and the boxes extend to 25 and 75 percentiles. Note that significance differences are only tested for males in the feminization experiments or females in the masculinization experiments.