REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-GFP (clone 7.1 and 13.1) antibody	Roche	Cat#11814460001; RRID: AB_390913
Mouse monoclonal anti-Hif1α (clone #241809) antibody	R&D Systems	Cat#MAP1536; RRID: AB_2116983
Rabbit polyclonal anti-CRY2	Bethyl Laboratories	Cat#A302-615A; RRID: AB_10554665
Mouse monoclonal anti-β-Catenin	BD Biosciences	Cat#610154; RRID: AB_397555
Rabbit polyclonal anti-Skp1 antibody	Cell Signaling	Cat#2156; RRID: AB_2270271
Rabbit polyclonal anti-Skp2 antibody	Cell Signaling	Cat#4313; RRID: AB_2187641
Rabbit polyclonal anti-Cul1 antibody	Invitrogen	Cat#71-8700; RRID: AB_87955
Rabbit monoclonal anti-β-TrCP (clone D13F10) antibody	Cell Signaling	Cat#4394S; RRID: AB_1054763
Mouse monoclonal anti-Sec31A (clone 32/Sec31A) antibody	BD Biosciences	Cat#612350; RRID: AB_399716
Mouse monoclonal anti-KLHL12 (clone 2G2) antibody	Thermo Fisher	Cat#MA5-15649; RRID: AB_11004907
Rabbit monoclonal anti-phospho-NF-κB p65 (Ser536) (clone 93H1) antibody	Cell Signaling	Cat#3033L; RRID: AB_331285
Mouse monoclonal anti-IκBα (clone L35A5) antibody	Cell Signaling	Cat#4814S; RRID: AB_10693636
Rabbit polyclonal anti-Cul2 antibody	Thermo Fisher	Cat#51-1800;RRID: AB_2533898
Mouse monoclonal anti-PCNA (clone PC10) antibody	Sigma-Aldrich	Cat#P8825; RRID: AB_477413
Goat polyclonal anti-ARIH1 antibody	Abcam	Cat#ab3891; RRID:AB_304129
Mouse monoclonal anti-HIF1α αντιβοδψ	BD Biosciences	Cat#610958; RRID:AB_398271
Rabbit polyclonal anti-NEDD8 antibody	Cell Signaling	Cat#2745S; RRID:AB_10695300
Rabbit monoclonal anti-p27 (Kip1) antibody	Cell Signaling	Cat#3686S; RRID:AB_2077850
Mouse monoclonal anit-Cyclin E1 antibody	Cell Signaling	Cat#4129S; RRID:AB_2071200
Anti-Flag M2 Magnetic Beads	Sigma	Cat#M8823
Mouse monoclonal anti-Tubulin antibody	Sigma	Cat#T9026; RRID:AB_477593
Rabbit polyclonal anti-CRY1 antibody	Bethyl	Cat#A302-614; RRID:AB_10555376
Chemicals, Peptides, and Recombinant Proteins
MG132 Proteasome inhibitor	InvivoGen	Cat#tlrl-mg132; CAS: 133407-82-06
TNF (murine recombinant)	Peprotech	Cat#315-01A
Tetracycline	Bioline	Cat#BIO-87030; CAS: 64-75-5
1,10-Phenanthroline	Sigma-Aldrich	Cat#131377-5G; CAS: 66-71-7
HALT protease and phosphatase inhibitor cocktail	Thermo Fisher	Cat#78441
Fluorescein-5-Maleimide	Thermo Fisher	Cat#F150
Tetramethylrhodamine-5-Maleimide (single isomer)	Thermo Fisher	Cat#T6027
Hoechst Solution	Thermo Fisher	Cat#62249
Blasticidin S HCl	Thermo Fisher	Cat#A1113903
Zeocin Selection Reagent	Thermo Fisher	Cat#R25001
Hygromycin B	Thermo Fisher	Cat#10687010
Puromycin Dihydrochloride	Thermo Fisher	Cat#A1113803
Roche mini complete EDTA free	Roche	Cat#11836170001
Roche complete	Roche	Cat#4693116001
phosSTOP inhibitor	Roche	Cat#04-906-837-001
1,10-Phenanthroline	Sigma	Cat#131377
MLN4924	EMD Millipore	Cat#5054770001
3x FLAG Peptide	LabPe	Cat#L1033
TMT10plex Label Reagent Set	Thermo Fisher	Cat#90406
TMT6plex Label Reagent Set	Thermo Fisher	Cat#90068
Trypsin Gold, MS grade	Promega	Cat#V5280
Lysyl Endopeptidase	Wako Chemicals	Cat#129-02541
Gateway LR Clonase Enzyme mix	Thermo Fisher	Cat#11791019
Experimental Models: Cell Lines
U2-OS	ATCC	ATCC#HTB-96; RRID:CVCL_0042
Flp-In T-Rex 293 Cell Line	Thermo Fisher	Cat#R78007; RRID:CVCL_U427
HEK293T	ATCC	ATTC#CRL-3216; RRID:CVCL_0063
Experimental Models: Organisms/Strains
E. coli BL21 Gold (DE3)	Thermo Fisher	Cat#50-125-348
E. coli Rosetta 2 (DE3)	EMD Millipore	Cat#71400
Sf9 Insect cells	Thermo Fisher	Cat#11496015
High Five Insect cells	Thermo Fisher	Cat#B85502
Recombinant DNA
pGEX4T1 GST-Thrombin-UBCH5B	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-UBCH7	Duda et al., 2013	N/A
pGEX4T1 GST-Thrombin-UBC12	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-UBE2F	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-APPBP1-UBA3	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-RBX1:His-Thrombin-CUL1CTD	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-RBX1:His-Thrombin-CUL1ΔWHB	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-RBX1ΔRING:His-Thrombin-CUL1CTD	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-RBX1(W35A):His-Thrombin-CUL1CTD	Scott et al., 2014	N/A
pGEX4T1 GST-Thrombin-RBX1(D36P):His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX1(I37A):His-Thrombin-CUL1CTD	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-RBX1(V38P):His-Thrombin-CUL1CTD	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-RBX1(V39G):His-Thrombin-CUL1CTD	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-RBX1(D40G):His-Thrombin-CUL1CTD	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-RBX1(N41G):His-Thrombin-CUL1CTD	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-RBX1(N47A:H48A):His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX1(D51A):His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX1(I54A):His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX1(Q57A):His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX1(F81N):His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX1(T90A:R91A):His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX1(V93R):His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX1(N98Q):His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX1(E102V):His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX1(F103V):His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX1(Y106I):His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX1(H108K):His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX1N-RBX2RING:His-Thrombin-CUL1CTD	This Study	N/A
pGEX4T1 GST-Thrombin-RBX2N-RBX1RING:His-Thrombin-CUL1CTD	This Study	N/A
pAL His-Thrombin-CUL1NTD	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-CKS1 (5-73)	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-NEDD8	Duda et al., 2008	N/A
pGEX4T1 GST-Thrombin-NEDD8 (Q40E)	This Study	N/A
pGEX2TK GST-Thrombin-UB (S > C)	Scott et al., 2014	N/A
pGEX2TK GST-Thrombin-UB (S > C) (I44A)	This Study	N/A
pGEX2TK GST-Thrombin-UB (S > C) (L71A)	This Study	N/A
pGEX2TK GST-Thrombin-UB (S > C) (L73A)	This Study	N/A
pGEX2TK GST-Thrombin-UB (S > C) (LR74A)	This Study	N/A
pGEX2TK GST-Thrombin-UBK0 (S > C)	This Study	N/A
pGEX4T1 GST-TEV-CDC34B	Duda et al., 2008
pGEX4T1 GST-TEV-CUL1CR3-C:His-MPB-TEV-RBX1 (CUL1 residues 332-C)	This Study	N/A
pGEX4T1 GST-TEV-CUL1CTD:His-MPB-TEV-RBX1 (CUL1 residues 441-C)	Jubelin et al., 2010	N/A
pGEX4T1 GST-TEV-SKP2101-C:SKP1	Duda et al., 2008	N/A
pGEX4T1 GST-TEV-FBW7ΔD:SKP1 (FBW7 residues 263-C)	Jubelin et al., 2010	N/A
pGEX4T1 GST-TEV CYCLINA	Duda et al., 2008	N/A
pGEX4T1 GST-TEV-CDK2:CIV1	Duda et al., 2008	N/A
pRSFDUET His-TEV-p27 (S10A:F62A:F64A)	Duda et al., 2008	N/A
pRSFDUET His-TEV-p27 K > R (All LYS Mutated to ARG; DNA insert synthesized by Open Biosystems)	Open Biosystems	N/A
pRSFDUET His-TEV-p27 K > R (S10A:F62A:F64A: K > R All LYS mutated to ARG)	This Study	N/A
pRSFDUET His-TEV-p27 K > R(R25K) (S10A:F62A:F64A)	This Study	N/A
pRSFDUET His-TEV-p27 K > R(R47K) (S10A:F62A:F64A)	This Study	N/A
pRSFDUET His-TEV-p27 K > R(R59K) (S10A:F62A:F64A)	This Study	N/A
pRSFDUET His-TEV-p27 K > R(R68K) (S10A:F62A:F64A)	This Study	N/A
pRSFDUET His-TEV-p27 K > R(R75K) (S10A:F62A:F64A)	This Study	N/A
pRSFDUET His-TEV-p27 K > R(R81K) (S10A:F62A:F64A)	This Study	N/A
pRSFDUET His-TEV-p27 K > R(R96K) (S10A:F62A:F64A)	This Study	N/A
pRSFDUET His-TEV-p27 K > R(R100K) (S10A:F62A:F64A)	This Study	N/A
pRSFDUET His-TEV-p27 K > R(R134K) (S10A:F62A:F64A)	This Study	N/A
pRSFDUET His-TEV-p27 K > R(R153K) (S10A:F62A:F64A)	This Study	N/A
pRSFDUET His-TEV-p27 K > R(R165K) (S10A:F62A:F64A)	This Study	N/A
pRSFDUET His-TEV-p27 K > R(R189K) (S10A:F62A:F64A)	This Study	N/A
pRSFDUET His-TEV-p27 K > R(R190K) (S10A:F62A:F64A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1	Duda et al., 2013	N/A
pGEX4T1 GST-TEV-ARIH190-C (ARIH1 residues 90-C)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (R118A:E119A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (V123D)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Q125)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (I130A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (H137A:N139A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (E143A:K144A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (E147A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Y149A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (F150A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N153A:L154A:E155A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K156A:L157A:F158A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (E160A:C161A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (H162A:V163A:I164A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N165A:P166A:S166A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K168A:K169A:S170A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (R171A:T172A:R173A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Q174A:M175A:N176A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (T177A:R178A:S179A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (I188A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (D241A:D242A:N243A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (T244A:V245A:M246A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (R247A:L248A:I249A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (T250A:D251A:S252A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K253A:V254A:K255A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K257A:Y258A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Q259A:H260A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (L261A:I262A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (T263A:N264A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (S265A:F266A:V267A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (E268A:C269A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N270A:R271A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (L272A:L273A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K274A:W275A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (P279A:D280A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (H282A:H283A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Q288A:Y289A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (D291A:K293A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (R296A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K298A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Q302A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (D313A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (W319A:L320A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K321A:K322A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (W323A:I324A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K325A:K326A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (C327A:D328A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (D329A:D330A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (S331A:E332A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (T333A:S334A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N335A:W336A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N340A:T341A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K342A:E343A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (P345A:K346A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (H348A:V349A:T350A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (I351A:E352A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N358A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (H359A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (M360A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (V361D)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (R363A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N364A:Q365A:N366A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K368A:E370A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (W373A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (V374A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (P378A:W379A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (E380A:P381A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (G383D:S384D:A385D)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (W386A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N388A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N390A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (R391A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Y392A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (D395A:D396A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (A397D:K398I:A399V)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (A400N:R401Q:D402S)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (A403Q:E405A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Q412A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (L415A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Y417A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N419A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (R420A:N423A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (H424A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (R429A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (F430A:E431A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (H432A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Y435A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (M455A:Q456A:Q457A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (W452A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K483A:K484A:N485A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Y476A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N486A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (I489A:I490A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (E492A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N493A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Q495A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (D497A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (E499A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N500A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K522A:Q523A:K524A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Q526A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (R530A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Y531A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (R535A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (R537A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (H452A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Y547A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (F430A:E431A:N503A) Open Mutant A	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (R420A:N423A:N503A) Open Mutant B	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (V123D:R420A:N423A:N503A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K156A:L157A:F158A:R420A:N423A:N503A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (I188A:R420A:N423A:N503A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (K257A:Y258A:R420A:N423A:N503A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (S265A:F266A:V267A:R420A:N423A:N503A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (N340A:T341A:R420A:N423A:N503A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (IT350A:I351A:R420A:N423A:N503A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (P378A:W379A:R420A:N423A:N503A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (W386A:R420A:N423A:N503A)	This Study	N/A
pGEX4T1 GST-TEV-ARIH1 (Y531A:W379A:R420A:N423A:N503A)	This Study	N/A
pMal MBP-TEV-KLHL12-His	Jin et al., 2012	N/A
pFastbac1 CUL1	This Study	N/A
pFastbacHTB His-TEV-CUL3	This Study	N/A
pFastbac1 SKP1	This Study	N/A
pFastbac GST-TEV-FBXL3	This Study	N/A
pFastbac GST-TEV-RBX1	This Study	N/A
pFastbac1 CRY1ΔC (CRY1 residues 1-532)	This Study	N/A
pFastbac GST-TEV-FBXW11	This Study	N/A
pFastbac SEC13	Jin et al., 2012	N/A
pFastbac His-TEV-SEC31A	Jin et al., 2012	N/A
pcDNA5 KLHL12-FLAG	Jin et al., 2012	N/A
pCS2 His-UB	Jin et al., 2012	N/A
pcDNA FRT/TO	Thermo Fisher	V652020
pDONR223 ARIH1	Orfeome V8.1	HsCD00378777
pOG44	Thermo Fisher	V600520
pHAGE-Puro-C-Flag-HA	Huttlin et al., 2015	N/A
pcDNA5-FRT/TO	Invitrogen	Cat#V6520-20
pcDNA5-FRT/TO+GFP	Kelsall et al., 2013	N/A
pcDNA5-FRT/TO+GFP-ARIH1	Kelsall et al., 2013	N/A
pcDNA5-FRT/TO+GFP-ARIH1 (C357S)	Kelsall et al., 2013	N/A
pcDNA5-FRT/TO+GFP-ARIH1 (siRNA resistant)	This study	N/A
Plasmid: pcDNA5-FRT/TO+GFP-ARIH1 (F430A, E431A, E503A)	Kelsall et al., 2013	N/A
Plasmid: pcDNA5-FRT/TO+GFP-ARIH1 (C357S, F430A, E431A, E503A)	Kelsall et al., 2013	N/A
Sequence-Based Reagents
siGENOME Non-targeting siRNA #1	Dharmacon	Cat#D-001210-01-05
Set of 4 upgrade: siGENOME ARIH1 siRNA; siRNA #1: GUGCUGGAGUGAAUAUUUA; siRNA #2: CGAGAUAUUUCCCAAGAUU; siRNA #3: ACACUUAUGUCUUCGCUUU; siRNA #4: GGAUAUGCCUUGUCAGAUC	Dharmacon	Cat#MU-019984-00-0002
Silencer Select Pre-Designed siRNA ARIH1: siRNA ID: s223658; siRNA #5: ACACGAAAUAGUGUGACCCat	Thermo Fisher	Cat#4392420
Silencer Select Pre-Designed siRNA ARIH1: siRNA ID: s223660; siRNA #6: UUCCAUCAAAGUACCUUUCca	Thermo Fisher	Cat#4392420
FlexiTube GeneSolution GS25820 for ARIH1; siRNA #7: CTGCTACTTGAACTACCCTAA; siRNA #8: CTGCGCTTTGAGCACAAACTA; siRNA #9: GCGCCTGATCACAGATTCAAA; siRNA #10: TAGAGTGCAATCGACTGTTAA	QIAGEN	Cat#GS25820
Software and Algorithms
Prism	GraphPad	ver 5; RRID: SCR_002798
R statistical package	http://www.rproject.org/	ver 3.2.3; RRID: SCR_001905
Bioconductor	https://www.bioconductor.org	ver 3.3; RRID: SCR_006442
Other
DMEM, high glucose, pyruvate	Thermo Fisher	Cat#11995-073
Lipofectamine 3000	Life Technologies	Cat#L3000008
Lipofectamine RNAi Max	Life Technologies	Cat#13778075
FxCycle PI/Rnase Staining Solution	Life Technologies	Cat#F10797
C-18 Empore extraction Disks	Thermo Fisher	Cat#14-386-2