Reference Table of Concentrations of Components in Biochemical Assays

Experiment (Fig Panel)	[E2~*UB] (μM)	[CRL] (μM)	[SR] (μM)	[Substrate] (μM)	[ARIH1] (μM)	pH	Temp (°C)
2B, S2A, S2B, 3A	0.4	0.4	0.4	0.6	0.3	7.5	23
S2D	0.4	0.4	0.4	0.6	Titrate	7.5	23
2C, 3B, S3C	0.4	0.4	0.4	2.0	0.3	7.5	23
2E	8.0	Titrate	Titrate	Titrate	0.01	7.5	23
S2E, S2F, 3C, S3D	0.4	0.4	0.4	0.4	0.3	7.5	23
3E, S3B	U7-0.4, 34-0.4	0.4	0.4	0.4	0.3	7.5	23
S2G	U7-0.4, U5-8.0, 34-8.0	0.4	0.4	0.4	0.3	7.5	23
S2H	U7-0.4, 34-Titrate	0.4	0.4	0.4	0.3	7.5	23
S3A	U7-0.4, 34-0.4	0.4	0.4	2.0	0.3	7.5	23
“Assay 1” 4C, S4A-I, S5H, 6B, S6A, 6H	0.4	0.4	0.4	2.0	0.3	6.5	0
“Assay 4” S5F, S5K, S5O, S5S, S5T, 6E	0.08	-	-	-	0.1	6.5	0
“Assay 5” S5G, S5L, S5P, S5U, S5V, 6C, 6J	0.08	0.1	0.1	0.5	0.06	6.5	0