| Digestion 1 | Digestion 2 | Digestion 3 | Digestion 4 | |
| Modified HBSS (ml) | 150 | 100 | 75 | 75 |
| DNAse (µl) | 300 | 200 | 150 | 150 |
| (0.1 mg/µl) | ||||
| MgSO4 (µl) | 150 | 100 | 75 | 75 |
| (800 mM) | ||||
| CaCl2 (µl) | 150 | 100 | 75 | 75 |
| (100 mM) | ||||
| Trypsin (U) | 1,824,000 | 1,200,000 | 960,000 | 960,000 |
| P/S (ml) | 1 | 0 | 0 | 0 |
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