Table 2.

The effect of seed-maturation light on germination responses to imbibition light in fresh seeds of each genotype imbibed at 10 and 22 °C

Geno-type	Type of imbibition response	Imbibition at 10 °C			Imbibition at 22 °C
		LR χ2	Effect	P-value	LR χ2	Effect	P-value
Ler	White light vs. dark	27·78	↓	<0·001	–	–	–
	White light vs. canopy	1·40	–	0·237	–	–	–
NIL	White light vs. dark	52·54	↓	<0·001	7·01	↑	0·008
	White light vs. canopy	0·01	–	0·954	6·59	↓	0·020
Col	White light vs. dark	0·35	–	0·557	1·50	–	0·220
	White light vs canopy	3·58	–	0·118	0·29	–	0·591

We tested whether maturation light modified two types of germination responses to imbibition light (Type of imbibition response): a light requirement for germination (White light vs. dark imbibition light) and germination response to an imbibition canopy (White light vs. canopy imbibition light). Germination proportions were analysed with logit-linked generalized linear models, and likelihood ratio (LR) tests were used to compare full models with reduced models that lacked the interaction between Maturation and Imbibition. Reference levels were white light for maturation and white light for imbibition. For each test, d.f. = 1 with 24 residual d.f. Arrows in the Effect column indicate that a maturation canopy significantly increased or decreased germination responses to imbibition light, as determined by the LR tests. Data separation prevented tests for Ler seeds at 22 °C, but these seeds responded to imbibition light treatments only if they were canopy matured (see Table S2).