Fig 4. ERα and KMT2B interdependently co-localized at IL-20 chromatin.

(A-B) ChIP analysis of KMT2B recruitment (A) and H3K4me1 enrichment (B) with or without siESR1(ERα knockdown) for 3 days or ICI treatment for 24 h, followed by E2 for 30 min. (C-D) ChIP assay showing the effect of KMT2B depletion on the recruitment of ERα (C) and RNA Pol II (D). The primers that amplify the ERα binding region of IL-20, i.e. segment2, were used in these experiments.