. 2016 Nov 2;11(11):e0164359. doi: 10.1371/journal.pone.0164359

Table 1. Overexpressed genes in all three sets.

Commonly overexpressed genes throughout the three test groups (25, 50 and 75 μg/ml Gentamicin Sulfate), where genes were up-regulated at least ten-fold in space with respect to their matched Earth (1g) controls in one or more of the tested conditions. S11 Table provides a Gene set analysis of these genes in pathways and with statistical significance.

Gene Name	Known or predicted function ^a	Molecular Function ^a	Biological Process ^a	25 μg/mL^b	50 μg/mL^b	75 μg/mL^b
entS_2	Enterobactin exporter EntS^*		enterobactin transport	2.36	14.44	2.45
gadX	HTH-type transcriptional regulator GadX	Catalytic activity / Nucleic acid binding	Metabolism / Biological regulation / Response to stimulus	13.73	5.82	2.05
hdeA	Acid stress chaperone HdeA			6.24	28.09	2.66
hdeB	Acid stress chaperone HdeB			6.10	29.08	2.68
hdeD	Protein HdeD			3.04	14.86	2.98
thiE	Thiamine-phosphate synthase	Catalytic activity	Metabolism / Cellular Process	2.40	28.59	6.57
thiF	Sulfur carrier protein ThiS adenylyltransferase	Catalytic activity	Metabolism / Cellular Process	2.41	28.87	7.44
thiG	Thiazole synthase			2.26	30.48	8.04
thiH	2-iminoacetate synthase	Catalytic activity	Metabolism	2.20	24.88	7.44
thiS	Sulfur carrier protein ThiS			2.49	32.41	6.01
yccJ	Uncharacterized protein YccJ			28.70	7.22	2.13
yegP	UPF0339 protein YegP			23.93	5.91	2.21
yhiD	Uncharacterized protein YhiD			6.67	19.57	2.16
yiaG	Uncharacterized HTH-type transcriptional regulator YiaG			14.22	8.45	4.36
yjdI	Uncharacterized protein YjdI			6.26	12.76	3.06

^a Based on the PANTHER (Protein ANalysis Through Evolutionary Relationships) Classification System database as of August 6, 2015 ref [48].

^b The fold-increase was acquired as the ratio of the average of the spaceflight samples expressions over the average of the Earth (1g) control samples expressions.

* This is the known or predicted function for the collective entS gene and not only for the entS_2 isoform.