Total RNA was isolated from spleens and brains from CC RIX mice infected with WNV for the indicated times. Plots show qPCR results for IFIT1 and IFN-ß expression (A) and WNV viral load in the spleen (B) and brain (C) as well as IFIT1, IFN-ß, and IL-12 expression in the brain (D) at innate time points, relative to mock values. Data represent 3 mice per group at d2, 7, and 12 time points in the discovery screen. Fig 3A & B, p<0.001 for all comparisons of CC(032x013)F1 to the other RIX lines at d2 (IFIT1 and IFN-ß); Fig 3C, p<0.01 for CC(032x013)F1 brain WNV load compared to the three non-disease lines at d7; Fig 3D, p<0.001 for all comparisons of CC(032x013)F1 to the other RIX lines at d2 (IFIT1); p<0.001 for all comparisons of CC(032x013)F1 to the other RIX lines at d7(IFN-ß and IL-12).