Fig. 3.

EndoG expression in human prostate cancer cell lines. (A) Expression of EndoG measured by using real-time RT-PCR. n = 4 per group, *p <0.001 compared to 22Rv1 and <0.05 compared to LNCaP cells. (B) Mn-dependent endonuclease (EndoG) activity in total protein extracts from the cells as measured using plasmid incision assay. O, open circular DNA; L, linear DNA; C, covalently closed supercoiled DNA, D, digested (fragmented) DNA. Each cell extract was tested using two concentrations of protein, 0.6 or 3 μg per sample. (C) Quantification of the plasmid incision assay. n = 4 per group, * p<0.001 compared to 22Rv1 or LNCaP cells.