Fig. 8.
Histone acetylation induced by TSA causes EndoG induction and increased sensitivity to cisplatin. (A) Histone acetylation induced by TSA caused EndoG induction only in EndoG-expressing 22Rv1 cells but not in EndoG-negative PC3 cells as determined by Western blotting. The membranes were stained with Ponseau S to control equal protein load (30 μg/well). Histone H4 acetylation was assessed using polyclonal acetyl-histone H4 antibody (Upstate, Lake Placid, NY). (B) Induction of EndoG by TSA (100ng/ml) in 22Rv1 cells was associated with the increased sensitivity to cisplatin (Cis, 25 μM).