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. 2016 Nov 2;11(11):e0165873. doi: 10.1371/journal.pone.0165873

Fig 3. Proteasome inhibition upregulates the major satellite transcription before any significant effect on the cell cycle distribution.

Fig 3

(A) Kinetics of expression of major and minor satellite repeats upon proteasome inhibition. NIH3T3 cells were treated with 20μM MG132 for 1h, 2h and 4h followed by RNA extraction and q-RT-PCR. The relative expression was normalised against spike and shows fold change relative DMSO. Error bars = SEM of at least 10 biological replicates. Proteasome inhibition upregulated the major satellite repeat expression at 4h of treatment, while minor satellite remained unaffected. (B) Treatment of cells with proteasome inhibitor MG132 for 4h does not significantly alter the distribution of the cell cycle profile. NIH3T3 cells were treated with DMSO or 20μM MG132 for 1h, 2h and 4h followed by PI staining and FACS analysis. Proportion (%) of acquired cells in G1/G0, S and G2/M phases are shown for treated and untreated cells. *p<0.05 (Student’s ‘t’ test).