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. 2016 Nov 2;11(11):e0165873. doi: 10.1371/journal.pone.0165873

Fig 4. HP1α delocalises from chromocentres upon proteasome inhibition whereas the histone modifications remain unaffected.

Fig 4

(A) Proteasome inhibition does not affect canonical histone modifications on major and minor satellite repeats. NIH3T3 cells were treated with 20μM MG132 for 4h followed by ChIP–qPCR analysis using antibodies against repressive mark H3K9me3 and activating marks H3K4me3, H3K36me3, and H3ac. Data is shown as relative enrichment to H3 with background subtraction. The y-axis scale was adjusted depending on the signal obtained with different antibodies. Error bars = SEM of 3 biological replicates. (B) Proteasome inhibition does not affect H3K9me3. NIH3T3 cells were treated with 20μM MG132 for 4h and immunolabeled with H3K9me3 antibody (red) and stained with DAPI (blue). Scale bar 10μm. (C) Delocalisation of HP1α from chromocentres upon proteasome inhibition. NIH3T3 cells were treated with 20μM MG132 for 4h and immunolabeled with HP1α antibody (green) and co-stained with DAPI (blue). Scale bar 10μm.