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. 2016 Aug 13;(114):54278. doi: 10.3791/54278
Component Stock concentration Final Concentration (in assay) Volume Comment
Dextran-647nm 75 μM 3.75 uM (0.6 μM) 5 μl
κ-casein 20 mg/ml 12.5 mg/ml (2 mg/ml) 62.5 μl
Tween-20 5% 0.375% (0.06%) 7.5 μl
BSA 200 mg/ml 12 mg/ml (1.9 mg/ml) 6 μl
MRB80 19 μl
100 μl final Store in 2 μl aliquots