Component | Stock concentration | Final Concentration (in assay) | Volume | Comment |
Dextran-647nm | 75 μM | 3.75 uM (0.6 μM) | 5 μl | |
κ-casein | 20 mg/ml | 12.5 mg/ml (2 mg/ml) | 62.5 μl | |
Tween-20 | 5% | 0.375% (0.06%) | 7.5 μl | |
BSA | 200 mg/ml | 12 mg/ml (1.9 mg/ml) | 6 μl | |
MRB80 | 19 μl | |||
100 μl final | Store in 2 μl aliquots |