Component	Stock concentration	Final Concentration	Volume	Comment
Blocking mix			1.6 μl
Tubulin	500 μM	30 μM	0.6 μl
Tubulin-488/561	50 μM	3 μM	0.6 μl
GTP	50 mM	5 mM	1.0 μl
Dynein-TMR	178 nM	30 nM	1.7 μl
Streptavidin	5 mg/ml	200 nM	0.4 μl	For cortical Dynein only
Kinesin-5	1.6 mM	80 nM	0.5 μl
ATP	25 mM	1 mM	0.4 μl	For motors only
PEP	0.5 M	25.6 mM	0.5 μl	For motors only
PK/LDH	800 U/1,100 U	23 U/32 U	0.3 μl	For motors only
Ase1-GFP	400 nM	80 nM	2.0 μl
Glucose	2.5 M	50 mM	0.3 μl	Last step
Glucose-oxidase	50x	1.0x	0.3 μl	Last step
MRB80			x
			9 μl final