Fig. 3.

Mutations of LXRE1, LXRE2, and SRE in SREBP-1c promoter sequence reduce insulin-induced activation in primary rat hepatocytes. The indicated WT or scramble mutant plasmid was transfected into hepatocytes, incubated with or without 100 nM insulin, and assayed for dual luciferase activity as described in the legend to Fig. 1. Each value represents the mean ± SEM of three independent transfection experiments (each assayed in duplicate). In each experiment, all of the mutant plasmids were studied. The normalized luciferase activity of the WT promoter in the absence of insulin was arbitrarily assigned a value of 1.0. The numbers above the bars refer to the fold increase by insulin.