Table 1. Insecticidal activity of spider venom components.
| Venom components | Physiological venom concentration, mM | LD50 concentration | Confidence limits, 95% | Synergistically tested concentration |
|---|---|---|---|---|
| CSTX-1 | 1.4-3.3 | 6.3 μM | 5.2-7.6 | |
| CSTX-9 | 0.2-1.1 | 211 μM | 190-273 | |
| CSTX-13 | 0.2-0.4 | 309 μM | 275-514 | 0.01-0.7 μM |
| Cupiennin 1a | ≈1.2 | 106 μM | 75-149 | 9.6 μM |
| Taurine | 0.07 | >160 mM | 0.07 mM | |
| Histamine | 5.7 | 917 mM | 794-1,100 | 5.7 mM |
| KCl | 215 | 1,845 mM | 1,650-2,131 | 215 mM |
Estimation of the lethal doses (LD50) in a Drosophila bioassay, where 50% of the test flies died of intoxication 24 h after injection. Different amounts of peptides, histamine, taurine, and KCl were dissolved in 0.1 M ammonium acetate, at a pH of 6.1, and 0.05 μl was injected into the flies. The physiological concentrations of CSTX-1 (13), taurine (13), histamine (13), KCl (13), CSTX-9 (15), and cupiennin 1a (12) in the venom were reported.