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. 2016 Nov 2;11(11):e0165926. doi: 10.1371/journal.pone.0165926

Fig 8. hCDC exosomes reduce proliferation of cardiac fibroblasts without affecting collagen gene expression.

Fig 8

(A, B) Human quiesced cardiac fibroblasts cocultured with either Scr CDCs or KD CDCs showed no statistically significant difference in COL1 or COL3 gene expression by qRT-PCR following TGFβ stimulation (n = 3). Data normalized to unstimulated Scr CDCs. (C, D) Cardiac fibroblasts cocultured with Scr CDCs for 24 hours showed a significant reduction in cellular proliferation as assessed by BrdU staining (5 hour pulse) when compared with (C, E) cardiac fibroblasts cocultured with nSMase2 KD CDCs and media control. n = 3. Scale bar 200μm. *p<0.05 using one-way AVOVA (Tukey’s post hoc test). Data are presented as mean ± SEM. White arrows highlight cells that co-stain for DAPI and BrdU.