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. 2004 Jul 26;101(31):11275–11280. doi: 10.1073/pnas.0404311101

Table 3. ΔCp of binding for different xylosaccharides with mutants lacking binding residues.

ΔCp, cal/mol·K
Xylan
Enzyme (mutant) X2 X3 X4 X5 X6 Birch wood Beech wood Stacking in subsites Ka (X6),* M-1 × 104
XT6(E159Q) -2 -94 -233 -257 -380 -330 -360 +1, +2, +3 290
XT6(E159QY203A) 6 -13 -154 -141 -238 +2, +3 19
XT6(E159QW273A) 0 -85 -93 -89 -190 +1, +3 54
IXT6(E134Q) -1 -90 -253 -242 -244 +1, +2 49
XT6(E159QQ102A) -5 -118 -239 -267 -359 +1, +2, +3 38
*

Ka is for X6 at 30°C; values are from Tables 1 and 2.