Table 3. ΔCp of binding for different xylosaccharides with mutants lacking binding residues.
ΔCp, cal/mol·K
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Xylan
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Enzyme (mutant) | X2 | X3 | X4 | X5 | X6 | Birch wood | Beech wood | Stacking in subsites | Ka (X6),* M-1 × 104 |
XT6(E159Q) | -2 | -94 | -233 | -257 | -380 | -330 | -360 | +1, +2, +3 | 290 |
XT6(E159QY203A) | 6 | -13 | -154 | -141 | -238 | +2, +3 | 19 | ||
XT6(E159QW273A) | 0 | -85 | -93 | -89 | -190 | +1, +3 | 54 | ||
IXT6(E134Q) | -1 | -90 | -253 | -242 | -244 | +1, +2 | 49 | ||
XT6(E159QQ102A) | -5 | -118 | -239 | -267 | -359 | +1, +2, +3 | 38 |