Condition	0.5x	0.7x	1x	2x	2x*
DNA	1 μg	1.4  μg	2  μg	4  μg	4  μg
Reduced serum media	100  μl	70  μl	100  μl	100  μl	200  μl
Transfection reagent	3  μl	4.2  μl	6  μl	6  μl	12  μl
*Note: Recommended for exceptionally difficult construct. Caution must be taken, as this condition induces high cell death.