Cell Buffer
Volume	Reagent	Comments
9 ml	ultrapure DNase/RNase free water
1 ml	HBS (10x), pH 7.4, store at 4 °C:
	200 mM HEPES
	50 mM KCl
	450 mM NaCl
	20 mM CaCl2 - H2O
	10 mM MgCl2 - H2O
100 μl	20% D-glucose
15 μl	aprotinin (1 mg/ml in dH2O)	prevent degradation
15 μl	leupeptin (1 mg/ml in dH2O)	prevent degradation
100 μl	ascorbic acid (100 mM in dH2O)*	stabilize agonist
	*add immediately before beginning assay
Drug Buffer
Volume	Reagent	Comments
9 ml	ultrapure DNase/RNase free water
1 ml	HBS (10x), pH 7.4, store at 4 °C:
	200 mM HEPES
	50 mM KCl
	450 mM NaCl
	20 mM CaCl2 - H2O
	10 mM MgCl2 - H2O
100 μl	ascorbic acid (100 mM in dH2O)*	stabilize agonist
	*add immediately before beginning assay